There are weight limitations associated with hovering and vertical landings. The F-35B, as I understand it, requires a A/C gross weight under 40,000 lbs in order to safely do this. This roughly translates to no stores aboard the airplane, as well as an internal fuel load of only 40% or so of total capacity. Harrier variants will have similar restrictions as well in regards to when a vertical takeoff or landing may be performed.
As the airplane will be operating close to full thrust during hover and/or vertical landing, it is consuming fuel at a great rate, so hovering operations are limited, generally only do a couple of minutes during final approach and landing during shipboard operations. At terrestrial airports, the F-35B and Harrier generally perform conventional landings. I also know, at least in the case of the F-35B, there are concerns about using the vertical lift system at terrestrial airports due to the risk of hot exhaust gas either cracking or damaging runways or parking aprons.
Due to the complexity of the lift fan system, an F-35B does not use it for anything aside from STOVL operations. There may be speed limitations as well with engaging the lift fan system on that aircraft, possibly somewhere in the area of below 250 KIAS. Harriers, however, could use nozzle position during all aspects of flight, including combat. Marine Corps tests performed in the 1970s in both computer simulations and on actual aircraft revealed the potential for using VIFF (Vectored thrust In Forward Flight) for both offensive and defensive maneuvering of the jet.