Has there ever been a landing made by an F-16 without the speedbrakes extended?
Here's the documentation from Lockheed on the F-16. From just a general glace, it looks like the only landing distance performance tables that are provided are for short-field operations (similar to how Cessna produces their POH), which all indicate that speedbrakes are extended.
Additionally, the same document lists "Speedbrakes - Close" in the after landing checklist, where it lists "Speedbrakes - Closed" in the before takeoff checklist. Deduce what you will from that, but to me this means the speedbrakes would have to be open for landing necessitating the 'close' action, not a verification that they are closed, as in the before takeoff checklist.
(Just as a side note, a podcast that I listen to quite regularly interviewed an F-16 pilot (John "Rain" Waters,) and he mentions in the podcast that the airplane was challenging to land because of its inherent desire to fly. So it would make sense then that in order to attain Vref or similar, speedbrakes would need to be extended. And it seems that way from the documentation as well.)
So, to directly answer your question: under normal procedures, no probably not.